Notes from the Field: Monitoring the Eastern Massasauga Rattlesnake

The Detroit Zoological Society (DZS) leads the Eastern massasauga rattlesnake Species Survival Plan (SSP), which is a comprehensive management plan through the Association of Zoos & Aquariums (AZA) that works to ensure the sustainability of healthy, genetically diverse and demographically varied captive animal populations. The DZS is also one of several organizations within the AZA that participates in a long-term study of a particular population of Eastern massasauga rattlesnakes in Michigan.

The Eastern massasauga rattlesnake is the mitten state’s only venomous viper, and is listed in Michigan as a species of special concern, which means it is threatened or endangered throughout its range. This SSP is a special one because each year, representatives from participating zoos attend a meeting in conjunction with an “in situ” study, which means that it takes place in the field.

This year marked our eighth monitoring a particular population of Eastern massasauga rattlesnakes in the southwest part of the state, and it was our best year to date – we found more than 100 snakes! This includes snakes that were found in previous years as well as newly identified individuals. The process involves gathering information on each snake in the field and then taking it to a lab where it is weighed and measured. We also determined if it is a male or a female and if female, whether it is pregnant (massasaugas give birth to live young). If the snake has never been found before, it is marked with a transponder tag – similar to those implanted in your pet dog or cat – so we can scan the animal and take measurements if it is found in the future. After the information has been gathered in the lab, the snake is then returned to the exact location where it was found.

Monitoring a seemingly healthy population over time gives us insight into natural fluctuations of the population size, male-to-female sex ratio, individual growth rates and reproductive success. As years goes by, the data will also begin to tell us life history data such as longevity of the species and how old animals remain reproductively active. All of this information assists the AZA zoos in how they manage the captive population as well as the state departments of natural resources in their management of the wild populations and the lands on which they are found.

– Jeff Jundt is the curator of reptiles for the Detroit Zoological Society and oversees the Holden Reptile Conservation Center at the Detroit Zoo.

Notes from the Field: Wolf-Moose Project on Isle Royale

Isle Royale is a remote wilderness island in northern Michigan, and at more than 40 miles long, it is the largest island in the largest Great Lake: Lake Superior.

I recently spent some time on Isle Royale working on a conservation research project called the Wolves and Moose of Isle Royale, along with Brian Manfre, a mammal department supervisor for the Detroit Zoological Society. Moose first came to Isle Royale in the early 1900s; wolves joined in the 1940s after crossing an “ice bridge” from Canada. For more than 50 years, the wolf-moose research project has studied the predator-prey dynamics of these species, making it the longest continuous study of any predator-prey system in the world. I was very eager to see the island and participate in the work first-hand.

Unfortunately, the wolf population at Isle Royale has dropped to only two closely related individuals, and the project now focuses on how the moose population is responding to reduced wolf predation. There is a proposal to reintroduce more wolves, but this is a complicated issue, in part because Isle Royale is also a National Park. The National Park Service will decide on this proposal in the fall of 2017.

Brian and I were joined by eight others working on this project. In addition to looking for moose bones to study the moose population, our team would also be investigating the presence of wolves at two previously used wolf dens. We set off together on a four-hour ferry ride from Copper Harbor to Isle Royale, and reported to the Bangsund cabin, an old fishing cabin that was built in 1926 and has served as the base for the project since 1959. We loaded our backpacks with hearty camp fare such as oatmeal, cheese, peanut butter and other supplies for the week, and then took a short boat ride to our departure site. After a 2-mile hike, we set up the first camp.

We camped at several different sites on the shores of Lake Superior and several inland lakes, and soon got into a routine of cooking, cleaning and filtering drinkable water at the camps. We were fortunate to have very pleasant weather. There was frost one of the first nights, but later in the week it warmed up enough for black flies to pester us.

On a usual day, we would hike 2-3 miles on-trail and then walk another 3-5 miles off-trail more slowly looking for moose bones. It was difficult at times, as we were going through dense forest, up and down ridges and through swamps. When a moose bone was spotted the requisite, “Bone!” was shouted out, and everyone would converge on the site to look for more bones. We were happy to find any bones at all, but skulls and teeth were especially prized. When cut in a cross section, the rings of dentin in the teeth can be counted to estimate the age of the moose – similar to counting tree rings – which can provide important information on the moose population. We can also examine vertebrae for signs of arthritis: Many of the moose at Isle Royale have been found to live longer and develop more arthritis than moose in areas with more predators. While it was a thrill to find these moose remains and evidence of wolves chewing on the bones, it was even more exciting to visit the old wolf dens. Unfortunately, there was no evidence of recent wolf use but we did find approximately one-week-old wolf feces along a trail nearby.

After a week, we returned to the Bangsund cabin with heavier packs than we left with, as they’d been loaded with moose skulls and other bones. We enjoyed a shower via bucket and ladle and then feasted on lasagna, wild rice casserole and a very welcome salad. We told stories and sang songs – it was a fitting celebration for a meaningful contribution to the project.

If you’re interested in participating in the wolf-moose study, visit this website.

– Paul Buzzard, Ph.D., is the director of conservation for the Detroit Zoological Society.

Animal Welfare: What, How and Why

I have written a number of blog entries on the animal welfare research projects we are conducting through the Detroit Zoological Society’s Center for Zoo Animal Welfare, and how collaborations enable us to move forward with many of the initiatives we undertake. Let’s now go back to the basics and explore what animal welfare is, how we go about evaluating the welfare of individual animals, and why this is fundamentally important.

The Association of Zoos & Aquariums’ Animal Welfare Committee defines animal welfare as an animal’s collective physical, mental and emotional states over a period of time, and is measurable on a continuum from good to poor. Although there are a number of other definitions available, the main factors remain consistent: Welfare is measured at the level of the individual animal, it encompasses all aspects of an animal’s life, and it can change over the course of time. The goal for anyone working with and around animals is to ensure that they each experience good welfare.

Going back many decades, people have long been concerned with the welfare of animals. In the 1960s, the Five Freedoms model was developed, originally as a means to assess the welfare of farm animals. This model states that animals should experience freedom from thirst, hunger and malnutrition, freedom from discomfort, freedom from pain, injury and disease, freedom from fear and distress, and freedom to express normal behavior. Since its initial development, this model has been applied in a variety of settings, including in zoos. However, the Five Freedoms model can be improved upon, as it is focused on minimizing negative states rather than also promoting positive welfare. Additionally, some of what is stated can be counter-productive to an animal’s survival. For example, if an animal never experiences thirst, then it may never drink, and this would not be a good thing. Therefore, the absolute freedom from some of the experiences is not even feasible. Rather, the important factor is ensuring that the resources necessary to perform the associated behaviors are available.

More recently, the Five Domains model was created, which delineates how nutrition, physical health, behavior and the environment (both physical and social) feed into an animal’s emotional state. The outcome is the individual’s welfare status. For example, if an animal is hungry but does not have access to food, this will result in a feeling of hunger, which will be a negative factor in the overall welfare status of that animal. If an animal is able to express natural behaviors, he or she will experience satisfaction, which is a positive emotion and contributes to positive welfare. All physical influences are taken into consideration as well as how they impact the internal, emotional state of the animal, in order to assess overall well-being.

Assessing welfare is a complex process that requires an understanding of the needs of a species and an individual as well as experience with scientific methods. It also typically includes multiple types of measures such as behavioral and physiological indicators. One can begin by evaluating what is made available to an animal, such as the physical space, the type of food presented and the social opportunities provided. This kind of assessment is known as a resource-based assessment, as it focuses on what we provide to the animals. To truly understand how an animal is faring, however, we also need to understand how they respond to their environment, and as such, conduct animal-based assessments. In our case, we usually observe how animals are interacting with their physical environment, with one another if they are a social species, and we utilize various physiological measures such as body condition, overall health and even hormone levels.

In order to ensure animals living in zoos are thriving, we need to understand what matters to them and that requires us to figure out how to “ask” them. Using existing methods and developing new ones to assess welfare is critical if we are to make evidence-based decisions for caring for animals. By letting animals tell us what is working and what needs to be improved, we are making their welfare a priority, and this is the ultimate responsibility we have to each and every animal living in the care of humans.

– Dr. Stephanie Allard is the director of animal welfare for the Detroit Zoological Society and oversees the Center for Zoo Animal Welfare.

Amphibian Conservation: Crawfish Frogs in Crisis

What is the best way to repurpose a basement in one of the buildings at the Detroit Zoo? Turn it into an amphibian’s paradise!

Amphibians across the globe are in crisis, with populations declining rapidly due to climate change, habitat destruction and a disease called the chytrid fungus. In an effort to reverse this trend for the crawfish frog (Lithobates areolatus), which is listed as Endangered in the state of Indiana, the Detroit Zoological Society (DZS) partnered with the U.S. Fish and Wildlife Service, the Department of Natural Resources and staff at Indiana University. In 2013 these entities began a repatriation project, which is an effort to return a species to its natural habitat. This involved the collection of egg masses from grasslands in Indiana, which were then transported to the Detroit Zoo, where staff raised them until they were almost froglets before releasing them back into the wild.

The crawfish frog can lay egg masses with as many as 5,000 eggs. That might make one wonder how these animals could be endangered, right? Well, even if all of those eggs hatched into tadpoles, less than one percent of those tadpoles would ever make it out of the water. Tadpoles are an easy prey for a wide variety of animals including birds, fish, mammals, frogs and even larger tadpoles. And even fewer tadpoles would make it far enough into adulthood to breed. This shows how vulnerable the tadpole stage is in a frog’s life, which is why we focused our efforts on this stage.

We knew what we needed to do, but where is there enough room for thousands of tadpoles at the Detroit Zoo? How about right under the noses of Jasiri and Tamba, two Southern white rhinos! Not directly under them, since 12,000 pounds of rhino would be harmful to a tadpole, but rather in the basement of their indoor habitat. It took a lot of hard work from staff, but the space was eventually turned into a fantastic temporary home for crawfish frog tadpoles. Some of their luxury items include manufactured ponds with an automatic rain system, spotlights and temperature control systems – and of course, plenty of food.

In April, the crawfish frog egg mass is transported from Indiana to the Detroit Zoo in a cooler. We take this egg mass right down to the basement where it is placed in water. In a couple of weeks, tadpoles start to emerge, which are then counted and moved into long pond-like pools. We raise these newly hatched tadpoles for about three months, with the amphibian department staff feeding and cleaning them daily. It takes hours to care for the tadpoles each day, but it is all worth it in the end when they are driven back to Indiana and released. The total number of tadpoles varies from year to year, but since this project started in 2013, the DZS has released a total of 10,411 tadpoles back into the wild.

While wild tadpoles have less than a one percent chance of survivability, we were able raise that to an astounding 99 percent in 2014 and 2015 for these “basement” tadpoles. That’s because they don’t need to worry about predators, disease, lack of food or pollution. This lets them get a good head start on life – but we don’t make it too easy for them. Otherwise, they wouldn’t know what to do once they got back to the wild. So before they fully metamorphose – or turn into frogs – they are taken back to Indiana and released into the ponds. Since some frogs lead a “double life” – aquatic and terrestrial – they are able to finish their aquatic life stage in enclosed repatariums, or screened-in containers, that allow protection before they are released as full frogs in the environment in which they will live. This method works well and provides exposure to the environment they will be living in before they become too accustomed to the luxury of the rhino basement.

The DZS crawfish frog conservation project requires long hours, but it is also the best time of the year as a member of the amphibian department staff. Seeing how the DZS can make a positive impact on this species and the ecosystem is very rewarding.

– Michael Andrus is an amphibian department zookeeper for the Detroit Zoological Society and works in the National Amphibian Conservation Center at the Detroit Zoo.

Veterinary Care: The Evolution of Zoo Vet Resources

I’ve been a practicing zoo veterinarian for 25 years; the last 20 of which have been spent here at the Detroit Zoo. Things have changed so much over the years, especially as it relates to the resources that are available to zoo and wildlife veterinarians as well as the technology we use to share information.

My first “real” job as a zoo veterinarian was at the Potawatomi Zoo in Indiana. I was the zoo’s first full-time veterinarian, and had to build the veterinary programs from scratch. I was a brand-new vet, full of ideas, and the only one at the zoo. It was a fun challenge and I had a lot to learn.

It was a very different time. When I first started I didn’t have a computer that was my own – I shared a very old model with our animal staff that did not have an internet connection. There was no email available back then; in order to reach out to someone for feedback or advice, you had to talk to them on a phone (that was connected to the wall!) The internet was not yet flush with information about zoo medicine, and most publications had to be rooted out of university libraries or actually found on your bookshelf.

So I made a lot of phone calls, talked to people at meetings, eventually sent a lot of emails, and connected with people on “listserves”. Some of my most valuable connections were within a group called the Midwest Regional Zoo Vets. This group started gathering soon after I started working in Indiana, and I very happily joined their ranks. It was a small group at first; five to seven people meeting to talk about anesthesia challenges and vaccine programs and sharing a spaghetti dinner made together in the hospital kitchen. I was the newest, youngest veterinarian in the group and found it invaluable to have this group as a resource.

Things have changed over the years. Electronic resources and connections make it much easier to keep the latest research at our fingertips and obtain feedback from other experts in our field. Despite all these resources, it’s still incredibly valuable to gather together and talk about the challenges we face. I’m happy to report that the Midwest Regional Zoo Vet group is still going strong after more than 23 years, and for most of these I’ve been the organizer and chairperson. We meet twice each year, rotating our locations between a dozen zoos in Ohio and the surrounding states. We have been able to maintain the intimacy of those first, small, cozy meetings, even though our last meeting was 30 members strong.

We talk about all kinds of things, from parasites to grief management, nutrition to radiology. We also often share clinical cases and invite outside experts to join in on the discussion. We talk about the cases that stump us, share our amazing success stories, and discuss our failures. There is no question that can’t be asked, no problem too trivial. I always learn so much more than I expect at our meetings – it’s fantastic! Zoo veterinarians are some of the most generous, dedicated people I know, and bringing my colleagues together to help provide the best possible medical care to animals is extremely rewarding. I’m proud to be part of a profession that feels like family!

– Dr. Ann Duncan is the Director of Animal Health for the Detroit Zoological Society and oversees the Ruth Roby Glancy Animal Health Complex.

Amphibian Conservation: Wyoming Toads Prepare for Breeding

A pair of small gold eyes peers out from under a mass of sphagnum moss. In a day or two, this warty creature will complete its climb out from the depths of the sandy soil where he has spent the last 30 days sleeping and “chilling” at a cool 38 degrees. Along with seven other individuals of this species, he will awaken due to an increase in temperature and light. By the time the temperature reaches 74 degrees, he will have completely removed himself from the soil and be sitting atop it, hopefully alongside his companion, a slightly larger female. In the week that follows, events these two small cold-blooded creatures will go through could lead to producing thousands of their species in a matter of hours due to their “explosive” nature.

The Wyoming toad (Anaxyrus baxteri) is one of North America’s most endangered species and one of the amphibians we work with as part of a Species Survival Plan (SSP) at the Detroit Zoo’s National Amphibian Conservation Center.  This toad’s wild populations began declining in the 1970s, and by the 1980s was listed as an Endangered Species by the International Union for the Conservation of Nature (IUCN). Possible causes for the population decline are use of aerial pesticides to eliminate mosquitoes, habitat manipulation, and disease in the form of the amphibian chytrid fungus. Realizing a decline was occurring, the last remaining toads were collected and by 1994, all remaining wild-born Wyoming toads were believed to be entirely in captivity. The IUCN now describes the Wyoming toad as extinct in the wild, meaning any toad currently found in the wild has come from captive breeding, in which the Detroit Zoological Society (DZS) has been actively involved.

The Wyoming toad SSP is managed by the Association of Zoos and Aquariums (AZA). The DZS has been a member of this conservation program since 1995 and since 2001 has sent a total of 6,505 tadpoles and toadlets to the wild. All Wyoming toads are the responsibility of the U.S. Fish and Wildlife Service (USFWS), so members of the AZA work closely with them to follow all USFWS permit guidelines for keeping them in captivity. The goal of any captive breeding program is to produce healthy offspring for release into the wild with the goal of rebuilding them into a self-sustaining population. Each toad in captivity is identified and tracked, which helps to pair toads that will produce the most genetically diverse and healthy offspring. Breeding pairs of toads are chosen almost a year in advance so toads have plenty of time to move from one zoo to another to create the breeding pairs.

Although not as well known as Jabari and Kivuli, the giraffe couple at the Detroit Zoo, Wyoming toads Butler and Beverly are a very important couple of toads. They are part of a “bio-secure” population and will never be seen by the public, which ensures they will not accidentally release diseases, known or unknown, into the wild via their offspring. They are held with four other pairs of Wyoming toads in a special room away from all other amphibians, so they will remain free of diseases that could otherwise be found in species not native to the western region of North America.

At the AZA’s recommendation, Butler arrived at the Detroit Zoo last October to be paired with Beverly, who arrived in 2012. Both are now 3 years old, which is about middle aged for a Wyoming toad. In early April, they were placed together into a thermostat-controlled “hibernaculum”, which is used to cool the toads, simulating the hibernation period a wild Wyoming toad would go through during the winter. The toads are kept cooled for about a month to help prepare them for breeding; eggs or sperms may develop and mature as a result of this cooling. The toads are given plenty of sandy soil to bury down into and sleep in just as they would have in the wild, and the Zoo’s animal care staff checks in on them weekly to make sure they are doing well.

At the end of May, the temperature in the hibernaculum will be increased to 70 degrees Fahrenheit and the toads will begin to awaken. Once they have climbed out from the soil, we will place them back into regular husbandry for a few days where they will continue to warm up and eat. Recordings of male toads calling are played during this time to condition toads further for breeding. Within a few days of emerging from hibernation, Butler and Beverly will be placed into a breeding chamber with 2-3 inches of water. The pair will hopefully go into “amplexus” within a few hours, which happens when the male positions himself on top of the female using his front legs and squeezes her, encouraging her to lay eggs. He fertilizes the eggs as she lays them, usually in the dark, overnight. Butler and Beverly should lay thousands of eggs, which is typical of toad species. Described as “explosive breeders”, the more eggs they can lay the better the chance offspring have of surviving the elements, diseases and predators. Since this is a captive breeding, we can help the thousands of eggs that Butler and Beverly produce survive into strong, healthy tadpoles.

Approximately two to three weeks after eggs have hatched into thousands of hungry tadpoles, arrangements are made with the USFWS to ship the tadpoles to Wyoming, where they will be released into protected wetlands. These tadpoles will add to those previously produced in captivity sent yearly by the zoos that participate in this program and the USFWS.

The wetlands are monitored during the spring and summer as part of yearly field surveys done to track the health and population of the toads. This year we hope that Butler, Beverly and the three other pairs of Wyoming toads at the Detroit Zoo are able to contribute thousands of healthy tadpoles to the wild population.

– Rebecca Johnson is the associate curator of amphibians for the Detroit Zoological Society.

Humane Education: Helping Animals in Need

As animal lovers, when we come across an animal in need of help, we want to take action. But when we find an injured or orphaned critter, often times we’re not sure what to do.

Here at the Detroit Zoological Society, we receive emails and calls throughout the year from people who want to aid local wildlife. Although we wish we could provide sanctuary for all animals in need, we are unable to do so in nearly all cases. But we think it’s wonderful that so many people want to assist these sentient beings, so we’ve compiled a list of helpful resources.

The following links contain contact information for reputable organizations and rehabilitators that are able to provide support. Download the app and jot down the phone numbers found in the links. You can keep a copy on hand so that the information is available should a need arise.

  • Animal Help Now App – Find contact information for nearby emergency wildlife assistance across the U.S., including wildlife rehabilitators, rescues and veterinarians.
  • All Species Kinship Wildlife Hotline – (877) 596-7776

Remember if you come upon an animal that you think needs help, don’t address the situation without first reaching out to a professional for his or her advice. If ever you’re in doubt, call an expert.

– Lisa Forzley is a curator of education for the Detroit Zoological Society and oversees the Berman Academy for Humane Education. Learn more about the Academy, which was created to help people help animals and offers a broad range of engaging programs, at http://detroitzoo.org/education/humane-education.

Education: Penguin Center offers Wealth of Learning Opportunities

The Polk Penguin Conservation Center is not only a state-of-the-art facility for penguins – the largest of its kind in the world – but it also contains a wealth of educational information about Antarctic explorers, modern-day researchers, and the incredible, fragile ecosystems at the bottom of the Earth.

During a visit to this incredible facility, visitors first enter the South American Gallery and are “met” by Sir Ernest Shackleton and the crew of his legendary ship, the Endurance. Shackleton led the ill-fated 1914 expedition to complete a transcontinental crossing of Antarctica. The endeavor became a survival story when his ship became trapped, and eventually crushed, by ice in the Weddell Sea. A dock scene tells the incredible survival story of the Endurance crew.

As you descend the entrance ramps and venture further into the penguin center, you “board” Shackleton’s Endurance and cross the Drake Passage to Antarctica. You may be met with a calm sea at day, a starlit night sky or the Drake’s notorious rough seas. Continuing down the ramps, you cross the Antarctic Convergence, which occurs when the moist air above the Atlantic, Pacific and Indian Oceans meet the frigid air above the Southern Ocean. The mixing of warm and cold air causes the moisture to condense and create fog. As you make your way below deck, portholes show glimpses of Antarctic wildlife including orcas, krill and leopard seals.

After passing through the acrylic underwater tunnels and the Underwater Gallery, the path brings you to a world of ice. Spotlight on Science showcases the research of world-renowned polar ecologist and penguin expert Dr. Bill Fraser, head of the Polar Oceans Research Group, and the importance of sea ice for the Antarctic ecosystem. Food sources for many species in the Antarctic region require sea ice for survival. Understanding these ecological changes caused by the shifting formation of sea ice can help us protect ecosystems in the future.

Across the cavernous room, Ice Core Investigations allows guests to explore ice cores, an important tool in uncovering the history of the Earth’s climate. Ice cores are drilled and excavated from thousands of years of compressed snow that has turned to ice. The air pockets trapped between layers serve as a record of what gasses filled the atmosphere over time, allowing us to compare different periods of climate history.

Watch for calving glaciers as you climb the stairs to the Antarctic Gallery. A glacier is a very large piece of ice that is pulled across land by gravity like a slow conveyor belt. Reaching the ocean, ice breaks off from the rest of the glacier and falls into the sea during a process known as calving.

Just outside the Drake Passage Gift Shop, Focus on the Field features the Detroit Zoological Society’s own Matthew Porter, a bird department zookeeper who had the rare and extraordinary opportunity to spend the 2015-2016 austral summer doing field work for the Polar Oceans Research Group in Antarctica. Matthew studied adélie penguins, chinstrap penguins, gentoo penguins, brown skuas, south polar skuas and southern giant petrels.

Learning about the Antarctic ecosystem while journeying through Polk Penguin Conservation Center may compel you to want to help in some way. Before exiting the penguin center, visitors have the opportunity to Make a Difference in the Antarctic Gallery. The Make a Difference kiosks guide you in finding ways to help. Whether it is buying your groceries locally, changing your home lighting to energy-efficient light bulbs, or riding your bicycle to work, you can make a difference with every step you take. The machines allow for you to take a picture of yourself, which is then placed onto a digital card that includes your pledge as well as facts about sustainability and the hashtag #MakeADifference. The digital card is emailed to you, with the option of also posting to social media sites to share with your friends and family.

All those who pass through the Polk Penguin Conservation Center have the ability and opportunity to join us in our mission of Celebrating and Saving Wildlife and leave a lasting, positive impact on the Earth.

– Claire Lannoye-Hall is a curator of education for the Detroit Zoological Society.

Animal Welfare: Educational Partnerships that Improve Animal Well-Being

In a number of instances, the work we do to enhance the welfare of the animals at the Detroit Zoo is a result of collaboration and partnerships. One such partnership is with Madonna University. Students enrolled in a class that is part of the humane education curriculum join us each semester to undertake projects that will benefit the animals.

We work with animal care staff to select projects suited to each species and the students are responsible for learning about that species to better understand the impact that their project will have on the animals’ welfare. The students are divided into three groups and each group is assigned a particular project. The groups work with mentors from the Life Sciences division and over the course of several weeks, complete their project. On the last day, all of the students get the chance to see the results of all of the projects in order to gain more appreciation of the various ways in which we can positively impact animals living in the care of humans.

Over the course of the last few years, the Madonna University students have participated in projects benefiting warthogs, amphibians, giraffes, gorillas, reptiles, rhinos and birds living in the Free-Flight Aviary, to name a few. This year, the students will help us to modify habitats for giant anteaters, flamingos and kangaroos and wallabies in the Australian Outback Adventure. This will involve the creation of nesting areas for the flamingos, as well as planting shrubs for the anteaters and kangaroos in order to add more complexity to their environments and create additional areas of shade. These types of modifications are important to ensure that animals encounter novelty and have more opportunities to display species-typical behaviors.

This wonderful partnership is an example of how the Detroit Zoo provides educational and inspirational opportunities for students and is always finding ways to design engaging habitats for the animals.

– Dr. Stephanie Allard is the Director of Animal Welfare for the Detroit Zoological Society and oversees the Center for Zoo Animal Welfare.

Veterinary Care: Ultrasound Exams for Frogs

Spring is in the air, and as I hike and bike the trails in southeast Michigan I find myself excited to be immersed in the lovely chorus of spring peepers and tree frogs as they prepare for this year’s breeding season. This also means it won’t be long until it is amphibian breeding season at the Detroit Zoo! Each year, the veterinary team works closely with our amphibian staff to provide support for our amphibian breeding programs. Our curators and keepers set up special breeding areas and adjust the temperature, light cycles, humidity and water access to simulate conditions in the wild and encourage natural breeding. They even set up simulated rainfall and play tape-recorded sounds of breeding calls collected from the wild.

Even with all of this, some of our endangered amphibians need a little more help to breed successfully.   At the Detroit Zoo, we have over a decade of experience administering special hormones to Wyoming toads and Puerto Rican crested toads to help with breeding. We have been very successful with these programs; two years ago the amphibian staff sent 3,914 Wyoming toad tadpoles to Wyoming and last year they sent 22,571 Puerto Rican crested toad tadpoles to Puerto Rico for release into their native habitat. This is important work and we are proud to be able to help.

As zoo husbandry staff and amphibian reproductive experts have gained expertise, it has become clear that not all amphibians respond the same way to changes in their environment and established hormone protocols. Two years ago, we purchased a high frequency ultrasound probe, and we have found that we can monitor the appearance of the ovaries and follicles as they develop and mature within female frogs and toads. This provides a very powerful tool for understanding the impact of the husbandry and hormone treatments that we apply, and will allow us to establish assisted reproduction methods for other endangered species.

This season we have some exciting things planned, and have started the early work to check the females we are hoping to breed. Last week, we performed ultrasound exams on our three female giant waxy tree frogs, and were able to see that they are all developing large, follicle-filled ovaries. Based on the appearance of the follicles, we think that they will be ready to breed in the next few weeks to a month.

This week, we will be conducting ultrasound exams on our endangered Mississippi gopher frog females. Once we see their degree of follicle development, we’ll be able to plan a hormone and husbandry strategy for this year’s breeding season. Hopefully we will have exciting news to share in the coming months!

– Dr. Ann Duncan is the Director of Animal Health for the Detroit Zoological Society and oversees the Ruth Roby Glancy Animal Health Complex.